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Biologist II
Contract: Cambridge, Massachusetts, US span>
Salary Range: 34.00 - 39.00 | Per Hour
Job Code: 364805
End Date: 2025-11-08
Job Status: Expired
This Job is no longer accepting applications
Pay Rate: $34.00 to $39.00 per hour
Job Description:
- Perform and maintain human colon organoid culture, including passaging and plating for experimental use.
- Conduct cell-based in vitro assays using mammalian cell lines and primary human immune cells (e.g., myeloid cells, PBMCs).
- Test small molecules and biologics in cell-based assays for readouts such as cytokine release, proliferation, and gene expression.
- Execute molecular and cellular biology techniques, including flow cytometry (FACS), ELISA, MSD, HTRF, and qPCR.
- Process animal tissue ex vivo and isolate immune cells from human or animal tissues or blood as needed.
- Design, execute, and interpret experiments to validate hypotheses aligned with project objectives.
- Collaborate with internal teams to coordinate organoid and immune cell requirements.
- Implement and optimize new laboratory protocols and experimental approaches.
- Analyze, interpret, and present data clearly and concisely in group and individual meetings.
- Maintain accurate laboratory records and ensure compliance with research quality and safety standards.
- Hands-on experience with human organoid culture.
- Experience with in vitro assays using primary human immune cells (myeloid, PBMCs).
- Proficiency in molecular and cellular analysis techniques (qPCR, MSD, ELISA, flow cytometry).
- Strong technical, analytical, and data interpretation skills.
- Excellent communication and teamwork abilities in a collaborative environment.
- Minimum 2+ years of experience in a biotech or pharmaceutical industry setting.
- Proven expertise in cell culture, particularly handling primary epithelial and immune cells.
- Experience with cell-based assay development and immunoassay techniques (ELISA, MSD, HTRF).
- Familiarity with gene expression analysis (qPCR) and ex vivo tissue processing (preferred).
- Bachelor’s degree in Biology, Immunology, or a related life sciences field required.
- An advanced degree is preferred but not required.
- Creative and detail-oriented problem solver
- Flexible and adaptable to changing project needs.
- Strong organizational, communication, and data documentation skills.Demonstrated ability to work independently and collaboratively in a fast-paced research environment.
Benefits:
The Company offers the following benefits for this position, subject to applicable eligibility requirements: medical insurance, dental insurance, vision insurance, 401(k) retirement plan, life insurance, long-term disability insurance, short-term disability insurance, paid parking/public transportation, (paid time, paid sick and safe time, hours of paid vacation time, weeks of paid parental leave, paid holidays annually – These all are subject to applicable eligibility).
Job Requirement
- Organoids
- CellCulture
- PBMCs
- Myeloid
- Immunology
- Cytokines
- Proliferation
- qPCR
- ELISA
- MSD
- HTRF
- FlowCytometry
- FACS
- AssayDevelopment
- Immunoassays
- GeneExpression
- TissueProcessing
- CellIsolation
- DataAnalysis
- Experimentation
- Protocols
- Collaboration
- Communication
- Documentation
- Adaptability
- ProblemSolving
- Organization
- Innovation
- Biology
- ImmunologyResearch
- MammalianCells
- EpithelialCells
- InVitro
- Discovery
- Benchwork
- Teamwork
- Biotech
- Pharmaceutical.
Reach Out to a Recruiter
- Recruiter
- Phone
- Siddhi Kadakia
- siddhi.kadakia@collabera.com